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細胞凍存試劑
成功凍存細胞并從液氮儲存中復蘇的能力是細胞培養(yǎng)研究中最重要的方面之一。細胞凍存液作為凍存細胞時的液體環(huán)境，為細胞提供營養(yǎng)和保護作用，降低了冰點并提高了細胞膜對水的通透性。這使細胞內(nèi)的水分在凍結前透出細胞，防止或減少了冰晶對細胞的損傷。因此，細胞可以暫時脫離生長狀態(tài)，將其細胞特性保存下來，需要時直接復蘇就可以恢復其活性。傳統(tǒng)的細胞凍存液是使用培養(yǎng)基、血清和DMSO按照一定的比例混合而成，雖然成本較低，但具有成分復雜、批次差異大、需要程序性降溫等缺點。因此，無血清凍存液在其基礎上發(fā)展而來，克服了傳統(tǒng)凍存液的以上缺點，得到了越來越廣泛的應用。
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產(chǎn)品詳情

表1 比較傳統(tǒng)細胞凍存液和無血清非程序細胞凍存液的特點。



傳統(tǒng)細胞凍存液
無血清非程序細胞凍存液
成分
基礎培養(yǎng)基+血清+DMSO,血清成分復雜
化學組成明確，含有營養(yǎng)成分和保護劑不含血清
凍存液配制
現(xiàn)用現(xiàn)配
即用型，無需配制，4℃保存，即取即用
凍存操作方法
較復雜，需要程序性降溫，操作時間長
簡單快捷，直接放入-80℃即可，省時省力
復蘇存活率
一般(微量凍存時細胞存活率低)
高(微量凍存時細胞存活率也高)
細胞保存設備
液氮
液氮或者-80℃低溫冰箱
安全性
有血清來源病毒、霉菌和支原體等污染風險
無血清來源病毒、霉菌和支原體等污染風險
凍存細胞種類
適合含血清細胞凍存
適合各類細胞凍存，尤其是無血清馴化細胞，節(jié)省再馴化步驟
批次差異
批次差異大
批次差異極小
整板凍存
不可行
可行，且方便快捷

NEST?可為您提供廣泛的無菌過濾，經(jīng)過應用測試的細胞凍存試劑產(chǎn)品，為您在凍存和復蘇過程中最大限度地提高細胞活力，請參考下表快 速選擇適合您實驗需求的產(chǎn)品。


產(chǎn)品名稱
應用
貨號
無血清非程序細胞凍存液(無酚紅)
化學成分明確，含有糖類、氨基酸等營養(yǎng)物質(zhì)以及 DMSO 等多種保護劑組合，大大降 低了在凍存過程中冰晶對于細胞的損傷，有效提高細胞復蘇率和活力。不含血清和蛋 白成分，無任何動物源組分，減少外源因子和污染源，更加安全、穩(wěn)定。同時可省去繁 瑣費時的程序性降溫過程，可直接重懸組織后置于-80°C 保存，或次日轉(zhuǎn)移到液氮中。
211072
二甲基亞砜(細胞培養(yǎng)級)
無菌包裝，適用于細胞凍存，也可用作細胞給藥溶液的配制溶劑。
211081


產(chǎn)品信息
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