產(chǎn)品詳情
胰蛋白酶（Trypsin）是一種絲氨酸水解酶，它能夠把多肽鏈中賴氨酸和精氨酸殘基中的羥基側(cè)切段，水解細(xì)胞間的蛋白質(zhì)，破壞細(xì)胞間的連接，從而使組織或貼壁細(xì)胞離散成單個(gè)細(xì)胞。胰酶分散細(xì)胞的活性與組織或細(xì)胞的特性、胰酶濃度、溫度和作用時(shí)間有關(guān)，在pH8.0和37°C時(shí)，胰酶的作用能力最強(qiáng)。因此，在使用胰酶時(shí)，應(yīng)當(dāng)把握好濃度、溫度和時(shí)間，以免消化過度造成細(xì)胞損傷。一般常用胰酶的工作濃度為0.25%，而半貼壁細(xì)胞或?qū)σ让该舾械募?xì)胞常采用低濃度（0.05%）的胰酶進(jìn)行細(xì)胞消化。由于EDTA能夠螯合Ca2+和Mg2+，從而破壞細(xì)胞連接促進(jìn)細(xì)胞的解離，因此在胰酶溶液中常常會(huì)加入一定量的EDTA混合使用，以增強(qiáng)解離效果。對(duì)于EDTA可能會(huì)干擾后續(xù)的測(cè)試分析時(shí)，推薦選擇不含EDTA的消化液。具體請(qǐng)參考以下表格選擇合適的胰蛋白酶產(chǎn)品。
儲(chǔ)存：-20度，干冰運(yùn)輸
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