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細(xì)胞凍存試劑
成功凍存細(xì)胞并從液氮儲(chǔ)存中復(fù)蘇的能力是細(xì)胞培養(yǎng)研究中最重要的方面之一。細(xì)胞凍存液作為凍存細(xì)胞時(shí)的液體環(huán)境，為細(xì)胞提供營(yíng)養(yǎng)和保護(hù)作用，降低了冰點(diǎn)并提高了細(xì)胞膜對(duì)水的通透性。這使細(xì)胞內(nèi)的水分在凍結(jié)前透出細(xì)胞，防止或減少了冰晶對(duì)細(xì)胞的損傷。因此，細(xì)胞可以暫時(shí)脫離生長(zhǎng)狀態(tài)，將其細(xì)胞特性保存下來，需要時(shí)直接復(fù)蘇就可以恢復(fù)其活性。傳統(tǒng)的細(xì)胞凍存液是使用培養(yǎng)基、血清和DMSO按照一定的比例混合而成，雖然成本較低，但具有成分復(fù)雜、批次差異大、需要程序性降溫等缺點(diǎn)。因此，無血清凍存液在其基礎(chǔ)上發(fā)展而來，克服了傳統(tǒng)凍存液的以上缺點(diǎn)，得到了越來越廣泛的應(yīng)用。
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產(chǎn)品詳情

表1 比較傳統(tǒng)細(xì)胞凍存液和無血清非程序細(xì)胞凍存液的特點(diǎn)。



傳統(tǒng)細(xì)胞凍存液
無血清非程序細(xì)胞凍存液
成分
基礎(chǔ)培養(yǎng)基+血清+DMSO,血清成分復(fù)雜
化學(xué)組成明確，含有營(yíng)養(yǎng)成分和保護(hù)劑不含血清
凍存液配制
現(xiàn)用現(xiàn)配
即用型，無需配制，4℃保存，即取即用
凍存操作方法
較復(fù)雜，需要程序性降溫，操作時(shí)間長(zhǎng)
簡(jiǎn)單快捷，直接放入-80℃即可，省時(shí)省力
復(fù)蘇存活率
一般(微量?jī)龃鏁r(shí)細(xì)胞存活率低)
高(微量?jī)龃鏁r(shí)細(xì)胞存活率也高)
細(xì)胞保存設(shè)備
液氮
液氮或者-80℃低溫冰箱
安全性
有血清來源病毒、霉菌和支原體等污染風(fēng)險(xiǎn)
無血清來源病毒、霉菌和支原體等污染風(fēng)險(xiǎn)
凍存細(xì)胞種類
適合含血清細(xì)胞凍存
適合各類細(xì)胞凍存，尤其是無血清馴化細(xì)胞，節(jié)省再馴化步驟
批次差異
批次差異大
批次差異極小
整板凍存
不可行
可行，且方便快捷

NEST?可為您提供廣泛的無菌過濾，經(jīng)過應(yīng)用測(cè)試的細(xì)胞凍存試劑產(chǎn)品，為您在凍存和復(fù)蘇過程中最大限度地提高細(xì)胞活力，請(qǐng)參考下表快 速選擇適合您實(shí)驗(yàn)需求的產(chǎn)品。


產(chǎn)品名稱
應(yīng)用
貨號(hào)
無血清非程序細(xì)胞凍存液(無酚紅)
化學(xué)成分明確，含有糖類、氨基酸等營(yíng)養(yǎng)物質(zhì)以及 DMSO 等多種保護(hù)劑組合，大大降 低了在凍存過程中冰晶對(duì)于細(xì)胞的損傷，有效提高細(xì)胞復(fù)蘇率和活力。不含血清和蛋 白成分，無任何動(dòng)物源組分，減少外源因子和污染源，更加安全、穩(wěn)定。同時(shí)可省去繁 瑣費(fèi)時(shí)的程序性降溫過程，可直接重懸組織后置于-80°C 保存，或次日轉(zhuǎn)移到液氮中。
211072
二甲基亞砜(細(xì)胞培養(yǎng)級(jí))
無菌包裝，適用于細(xì)胞凍存，也可用作細(xì)胞給藥溶液的配制溶劑。
211081


產(chǎn)品信息
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