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DMEM高糖培養(yǎng)基
DMEM培養(yǎng)基（Dulbecco's Modified Eagle Medium）是一種廣泛使用的基礎(chǔ)培養(yǎng)基，適用于多種哺乳動(dòng)物細(xì)胞培養(yǎng)，包括原代成纖維細(xì)胞、神經(jīng)元、神經(jīng)膠質(zhì)細(xì)胞、HUVEC和平滑肌細(xì)胞，以及HeLa、293、Cos-7和PC-12等細(xì)胞系。DMEM是在MEM培養(yǎng)基的基礎(chǔ)上研制的，與MEM培養(yǎng)基相比，氨基酸的含量增加了 2 倍，維生素增加了 4 倍，同時(shí)還增加了非必需氨基酸、微量鐵離子以及丙酮酸鈉。DMEM培養(yǎng)基最初設(shè)計(jì)為葡萄糖含量1000mg/L的低糖型，后來又發(fā)展出葡萄糖含量為 4500mg/L 的高糖型，現(xiàn)已廣泛應(yīng)用于各種細(xì)胞的培養(yǎng)。DMEM 高糖型普遍應(yīng)用于生長快、粘附性低的細(xì)胞、雜交瘤的骨髓瘤細(xì)胞、克隆細(xì)胞、DNA 轉(zhuǎn)染的轉(zhuǎn)化細(xì)胞、原代病毒宿主細(xì)胞、單一細(xì)胞的培養(yǎng)以及疫苗的生產(chǎn)，例如利用CHO細(xì)胞表達(dá)EPO和生產(chǎn)乙肝疫苗。
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產(chǎn)品詳情
  1. 產(chǎn)品名稱
規(guī)格貨號(hào)
DMEM高糖培養(yǎng)基（含L-谷氨酰胺，丙酮酸鈉；不含HEPES，雙抗）
500mL/瓶
209011


該DMEM高糖培養(yǎng)基配方：

With含有
 Without不含
葡萄糖(4500mg/L)
·HEPES
L-谷氨酰胺(4mM)

丙酮酸鈉(110mg/L)

酚紅(158mg/L)

                                                                                                                        *更為詳細(xì)的配方請(qǐng)參考說明書。


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