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DMEM/F-12培養(yǎng)基
DMEM/F-12培養(yǎng)基（Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12）是DMEM培養(yǎng)基和Ham's F-12培養(yǎng)基的1:1混合物。它在DMEM培養(yǎng)基的基礎上添加了F-12培養(yǎng)基中更為豐富的營養(yǎng)成分，包括多種微量元素。DMEM是在MEM培養(yǎng)基的基礎上研制的，與MEM培養(yǎng)基相比，氨基酸的含量增加了2倍，維生素增加了4倍，同時還增加了非必需氨基酸、微量鐵離子以及丙酮酸鈉。Ham's F-12以Ham's F-10培養(yǎng)基為基礎，顯著提高了膽堿、肌醇、腐胺和幾種氨基酸的濃度。DMEM/F-12被廣泛用于支持多種哺乳動物細胞的生長，包括MDCK、神經(jīng)膠質(zhì)細胞、成纖維細胞、人內(nèi)皮細胞和大鼠的成纖維細胞等。同時，DMEM/F12常作為開發(fā)無血清培養(yǎng)基的基礎，也適用于低血清含量下哺乳動物細胞的培養(yǎng)以及降低細胞密度進行培養(yǎng)。

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產(chǎn)品名稱規(guī)格貨號
DMEM/F-12(含L-谷氨酰胺，丙酮酸鈉，HEPES）
500mL/瓶
209031

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